HLA-DRB1 Antibody (MHC II)

SKU: orb749569

描述

This mAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

研究领域

Cell Biology

图片与验证

−

Item 1 of 4

已测应用	FACS, IF, IHC-P, WB
稀释范围	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
反应性	Human
应用说明	Optimal dilution of the anti-HLA-DRB1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

−

抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2b, kappa
克隆号	SPM423
免疫原	Activated human peripheral blood mononuclear cells were used as the immunogen for the anti-HLA-DRB1 antibody.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

−

储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

−

Item 1 of 10
HLA-DPB1 Antibody [orb608003]
应用：
ELISA, FC, IF, IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 8
HLA-DPB1 Antibody [orb607962]
应用：
FC, IF, IHC, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 6
HLA-DRA Antibody [orb2312526]
应用：
IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 6
HLA-DRB1 Antibody [orb2312931]
应用：
IHC, WB
反应性：
Human
种属/宿主：
Rabbit
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 20 μg, 100 μg, 100 μg (without BSA and Azide)
Item 1 of 6
HLA-DRB1 Antibody (MHC II) [orb749570]
应用：
FACS, IF, IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.