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特色
有活性
描述
研究领域
Cardiovascular Research
图片与验证
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Item 1 of 7
| 应用说明 |
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关键属性
−| 来源 | Mammalian cell |
|---|---|
| 生物学来源 | Homo sapiens (Human) |
| 生物学活性 | ①Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of SARS-CoV-2 S1 protein, add 100μl/well (0.7629-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1 protein could bind ACE2 protein. The EC50 is 56.64 - 103.6 ng/ml. ②Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein, add 100μl/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 31.80 - 44.69 ng/ml. ③Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.5μg/well (5μg/ml, 100μl/well) of SARS-CoV-2 S1-RBD protein, add 100μl/well (0.762939-100000ng/ml, gradient dilution, 2x to 18x) of ACE2 protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 2.785-9.139 ng/ml. ④Measured by ACE2 protein binding ability in a functional ELISA. Coat the plate with 0.2μg/well (2μg/ml, 100μl/well) of ACE2 protein, add 100μl/well (0.00001164153-200000ng/ml, gradient dilution, 4x to 18x) of SARS-CoV-2 S1-RBD protein and 100μl/well of Peroxidase-Affinipure Goat Anti-Human IgG, Fcγ Fragment Specific. Wells were washed with buffer, and then A450 nm was measured. The results showed SARS-CoV-2 S1-RBD protein could bind ACE2 protein. The EC50 is 8.363-12.82 ng/ml ng/ml. ⑤The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein. ACE2 protein was injected at 5 concentrations (400, 200, 100, 50, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 100 nM. ⑥The SPR sensorgram shows the binding between ACE2 protein and SARS-CoV-2 S1-RBD protein. ACE2 protein was injected at 5 concentrations (100, 50, 25, 12.5, 0 nM) across 0.5 mg/ml of SARS-CoV-2 S1-RBD protein captured on a COOH chip. The results showed ACE2 protein could bind SARS-CoV-2 S1-RBD protein with an affinity constant of 13.8 nM. |
| 标签 | C-terminal hFc1-tagged |
| 表达区域 | 18-740aa |
| 蛋白长度 | Partial |
| 内毒素 | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 112.5 kDa |
| 纯度 | Greater than 85% as determined by SDS-PAGE. |
| 蛋白序列 | QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS |
储存与操作
−| 储存 | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| 形态/外观 | Lyophilized powder |
| 缓冲液/防腐剂 | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
| 有效期 | 6 months from date of receipt. |
| 免责声明 | For research use only |
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质量保证
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常用数据库链接
UniProt
UniProt 详情
−暂无 UniProt 数据
