ICG-001

SKU: orb1710780

描述

ICG001 is a selective small molecule inhibitor that disrupts the β-catenin/CBP interaction to block Wnt/β-catenin signaling, with an IC50 of 3 μM for CREB binding. It is a valuable research tool for studying oncogenic signaling in cancers, including colorectal carcinoma, in both cellular and animal models.

研究领域

Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号	780757-88-2
MW	548.63
纯度	>99.99% (May vary between batches)
分子式	C₃₃H₃₂N₄O₄
SMILES	Oc1ccc(C[C@@H]2N3[C@H](CN(Cc4cccc5ccccc45)C2=O)N(CCC3=O)C(=O)NCc2ccccc2)cc1
靶点	Wnt/beta-catenin,Apoptosis,Epigenetic Reader Domain
溶解性	2% DMSO+40% PEG300+5% Tween 80+53% Saline:10 mg/mL (18.23 mM);DMSO:242 mg/mL (441.1 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:9 mg/mL (16.4 mM)

生物活性

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靶点 IC50	CBP:3 μM
体内	METHODS: In a tumor regression SW620 nude mouse xenograft model, ICG-001 (150 mg/kg, intravenous injection) was administered to observe the effect of the drug on tumor remission in nude mice. RESULTS Tumor volume was significantly reduced during the 19-day treatment, with no mortality or weight loss.
体外	METHODS: SW480 cells were treated with ICG-001 (25 μM, 24 h) and the inhibition of survivin gene transcription was measured by semiquantitative RT-PCR; SW480 and HCT116 cells were treated with ICG-001 (10 and 25 μM, 24 h) and analyzed by immunoblotting for survivin; SW480 cells were treated with ICG-001 (25 μM) and the chromatin immunoprecipitation assay of the cyclin D1 promoter was used; promoter occupancy was assessed with CBP- (AC-22) or p300- (C-20) specific antibodies in the presence of ICG-001 (25 μM, 8 h). RESULTS After 8 h of treatment of SW480 cells with 25 μM ICG-001, approximately 2% of the genes analyzed were upregulated by a factor greater than 2, while only approximately 0.3% of the genes were downregulated by more than 50%. The two genes downregulated were S100A4 and survivin, which are the first and fourth mRNAs upregulated in cancer cells, respectively. ICG-001 effectively reduced the steady-state levels of survivin mRNA and protein in treated colorectal cancer cells. SW480 cells treated with ICG-001 (25 μM) showed a significant decrease in cyclin D1 as early as 4 h after treatment. Using chromatin immunoprecipitation, ICG-001 was shown to selectively inhibit the endogenous cyclin D1 promoter β.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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inhibit, EpigeneticReaderDomain, Epigenetic Reader Domain, Inhibitor, ICG 001, ICG001, ICG-001, betacatenin, beta-catenin, Beta catenin, CBP, 847591-62-2, bcatenin, Apoptosis, β-catenin, βcatenin, Wnt/β-catenin, Wnt/betacatenin, Wnt/b-catenin, Wnt

相似产品

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ICG-001 [orb1305968]
纯度：
>98%
CAS 号：
847591-62-2
MW:
548.63
分子式：
C₃₃H₃₂N₄O₄
可选规格： 25 mg, 5 mg
PRI-724 [orb1685665]
纯度：
99.11% (May vary between batches)
CAS 号：
1422253-38-0
MW:
658.64
分子式：
C₃₃H₃₅N₆O₇P
可选规格： 1 mg, 5 mg, 10 mg, 25 mg, 50 mg, 100 mg, 1 ml x 10 mM (in DMSO)
PRI-724 [orb1223869]
纯度：
>98% (HPLC)
CAS 号：
847591-62-2
MW:
548.63
分子式：
C₃₃H₃₂N₄O₄
可选规格： 5 mg, 25 mg, 100 mg, 500 mg, 1 g, 2 mg, 50 mg, 10 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

11238147 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	>98%
CAS 号：	847591-62-2
MW:	548.63
分子式：	C₃₃H₃₂N₄O₄

纯度：	99.11% (May vary between batches)
CAS 号：	1422253-38-0
MW:	658.64
分子式：	C₃₃H₃₅N₆O₇P

纯度：	>98% (HPLC)
CAS 号：	847591-62-2
MW:	548.63
分子式：	C₃₃H₃₂N₄O₄

ICG-001

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

ICG-001 [orb1305968]

PRI-724 [orb1685665]

PRI-724 [orb1223869]

质量保证

化合物标识

关键属性

实验方案信息

可选规格