MUC1 Antibody / Mucin-1 / EMA

SKU: orb605908

描述

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

研究领域

Immunology & Inflammation

图片与验证

−

Item 1 of 8

已测应用	IHC-P, WB
稀释范围	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
反应性	Human
应用说明	Optimal dilution of the MUC1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

−

抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2a, lambda
克隆号	MUC1/520
免疫原	Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

−

储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

−

Item 1 of 8
MUC1 Antibody / Mucin-1 / EMA [orb2638903]
应用：
IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg
Item 1 of 7
MUC1 Antibody / Mucin-1 / EMA [orb2638902]
应用：
IHC-P
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 7 ml

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.