MUC1 Antibody / Mucin-1 / EMA

SKU: orb2638902

描述

In Western blotting, it recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of MUC1/Mucin-1/Epithelial Marker Antigen/EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

研究领域

Immunology & Inflammation

图片与验证

−

Item 1 of 7

已测应用	IHC-P
稀释范围	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
反应性	Human
应用说明	Optimal dilution of the MUC1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

关键属性

−

抗体类型	Primary Antibody
宿主	Mouse
克隆性	Monoclonal
同种型	Mouse IgG2a, lambda
克隆号	MUC1/520
免疫原	Human milk-fat globule membranes (HMFGM) was used as the immunogen for the MUC1 antibody.
纯化	Protein G affinity chromatography
偶联	Unconjugated

储存与操作

−

储存	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
缓冲液/防腐剂	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
有效期	12 months from date of receipt.
免责声明	For research use only

相似产品

−

Item 1 of 8
MUC1 Antibody / Mucin-1 / EMA [orb605908]
应用：
IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg, 20 μg
Item 1 of 8
MUC1 Antibody / Mucin-1 / EMA [orb2638903]
应用：
IHC-P, WB
反应性：
Human
种属/宿主：
Mouse
克隆性：
Monoclonal
偶联：
Unconjugated
可选规格： 100 μg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

常用数据库链接

UniProt

P15941

UniProt 详情

−

暂无 UniProt 数据

实验方案信息

IHC-P

石蜡切片免疫组化

查看实验方案

查看更多实验方案