RNase A (DNase free)

SKU: orb1734799

描述

RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use.

图片与验证

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应用说明

Plasmid and genomic DNA preparation Removal of RNA from recombinant protein preparations Ribonuclease protection assays Mapping single-base mutations in DNA or RNA. Working concentration: 1-100 µg/ml (depending on application). The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids. At NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves ssRNA. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents. Isoelectric point (pI): 9.6. Optimal pH: 7.0 (activity range 6-10). Activity: ≥ 80 Kunitz units/mg. Unit definition: 1 Kunitz unit is that amount of activity which is capable of causing within 1 minute a decrease in absorbance at 300 nm equivalent to the maximum possible change in a 0.05% solution of yeast RNA at 25 °C, pH 5.0. Inactivation: Ribonuclease inhibitor, Vanadyl-ribonucleoside complexes, arabi-nonucleosides, Zn2+, Cu2+, penicillin, Vitamin B12, SDS, DEPC, 4 M guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol. Most polyan-ions show some inhibitory effect. Inactivated by phenol/chloroform extraction.

关键属性

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生物学活性	≥ 80 Kunitz units/mg
CAS 号	9001-99-4
MW	Theoretical MW: 13.7 kDa (monomer)
纯度	≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate

储存与操作

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储存	store at 2-8 °C. stock solutions in TE buffer should be aliquoted and stored at -20 °C
形态/外观	dry powder
有效期	6 months from date of receipt.
免责声明	For research use only

别名

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Endoribonuclease, Bovine pancreas

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RNase I (DNase free) [orb532705]
纯度：
> 95% (SDS-PAGE)
MW:
Theoretical MW: 27.0 kDa
可选规格： 5 x 2000 units
RNase A (DNase free) [orb532708]
纯度：
≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate
MW:
Theoretical MW: 13.7 kDa (monomer)
可选规格： 2 x 250 mg

质量保证

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实验方案信息

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纯度：	≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate
MW:	Theoretical MW: 13.7 kDa (monomer)

RNase A (DNase free)

描述

图片与验证

关键属性

储存与操作

别名

相似产品

dNTP Mix (PCR Grade) 10 mM each [orb782911]

DNase I [orb1736803]

Magbead Plant RNA Kit [orb3074002]

RNase I (DNase free) [orb532705]

RNase A (DNase free) [orb532708]

质量保证

实验方案信息

可选规格