RNase A (DNase free)

SKU: orb532708

描述

RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.

图片与验证

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应用说明	Applications: 1) Plasmid and genomic DNA preparation 2) Removal of RNA from recombinant protein preparations 3) Ribonuclease protection assays 4) Mapping single-base mutations in DNA or RNA.

关键属性

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生物学活性	≥ 80 Kunitz units/mg
CAS 号	9001-99-4
MW	Theoretical MW: 13.7 kDa (monomer)
纯度	≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate

客户验证数据

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反应性分布

最新实验

2 Results

稀释

样本

摘要

In vitro

Human

100 μg/mL

Fixed melanoma cell suspensions (SKMEL28, MALME3M, A375, G361, UACC62, MELST, HEK293FT)

RNase A was used in cell...

In vitro

Human

0.8 mg/mL

Cultured human cells (H1299 cell line)

RNase A was used as a...

储存与操作

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储存	store at 2-8 °C
形态/外观	dry powder
浓度	Working concentration: 1-100 µg/ml (depending on application). The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids.
有效期	6 months from date of receipt.
免责声明	For research use only

别名

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Endoribonuclease, Bovine pancreas

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纯度：
> 95% (SDS-PAGE)
MW:
Theoretical MW: 27.0 kDa
可选规格： 5 x 2000 units
RNase A (DNase free) [orb1734799]
纯度：
≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate
MW:
Theoretical MW: 13.7 kDa (monomer)
可选规格： 2 x 50 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

实验方案信息

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纯度：	≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate
MW:	Theoretical MW: 13.7 kDa (monomer)

RNase A (DNase free)

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

相似产品

dNTP Mix (PCR Grade) 10 mM each [orb782911]

DNase I [orb1736803]

Magbead Plant RNA Kit [orb3074002]

RNase I (DNase free) [orb532705]

RNase A (DNase free) [orb1734799]

质量保证

实验方案信息

可选规格