SP-600125

SKU: orb1304531

特色

描述

SP600125 is a potent, orally bioavailable, and reversible ATP-competitive inhibitor of JNK1, JNK2, and JNK3. It is widely used in research to study JNK signaling pathways, demonstrating effects on autophagy inhibition and apoptosis induction in both cellular and animal models of stress and disease.

研究领域

Cell Biology, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号	129-56-6
MW	220.2
纯度	>98%
分子式	C₁₄H₈N₂O
SMILES	O=C1C2=C3C(NN=C3C3=C1C=CC=C3)=CC=C2
靶点	Kinase
溶解性	Soluble in DMSO (up to 22 mg/ml) or in Ethanol (up to 2 mg/ml).

生物活性

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靶点 IC50	JNK3:90 nM\|JNK1:40 nM\|JNK2:40 nM
体内	METHODS: To test the inhibitory activity of TNF-α in vivo, SP600125 (7.5-30 mg/kg, 30% PEG-400/20% polypropylene glycol/15% Cremophor EL/5% ethanol/30% saline) was administered intravenously or orally to CD-1 mice 15 min after LPS-induced TNF-α expression was injected. LPS-induced TNF-α expression was injected 15 min later. RESULTS: Intravenous administration of 15 or 30 mg/kg SP600125 significantly inhibited TNF-α serum levels, while oral administration dose-dependently blocked TNF-α expression, with a significant inhibitory effect observed at 30 mg/kg per oral dose. METHODS: To test the antitumor activity in vivo, SP600125 (5 mg/kg) and C-2 (10 mg/kg) were injected intraperitoneally into nude mice bearing the bladder cancer tumor BIU87 once a day for twenty-one days. RESULTS: C-2 treatment inhibited tumor growth, and tumors in the C-2/SP600125 group were significantly lower than those in mice treated with vector or C-2 alone.
体外	METHODS: Mouse lung cancer cells LLC and mouse tumor cells 4T1 were treated with SP600125 (3-10 μM) for 72 h, and cell viability was detected using MTT assay. RESULTS: SP600125 dose-dependently inhibited the growth of LLC and 4T1 cells with IC50 of 8.14 μM and 7.37 μM. METHODS: Jurkat T cells were pretreated with SP600125 (1-50 μM) for 10 min, and then stimulated with PMA (50 ng/mL), anti-CD3 (0.5 μg/mL), and anti-CD28 (2 μg/mL) for 30 min, and then the expression levels of target proteins were detected by Western Blot. RESULTS: SP600125 blocked the phosphorylation of c-Jun at an IC50 of 5-10 μM. At a concentration of 50 μM, SP600125 did not block ERK phosphorylation or inhibit IκBα degradation. Partial inhibition of phospho-p38 and ATF2 was observed at 50 μM, but not at 25 μM.
细胞研究	Multiarray plate screening of mRNA was performed by High Throughput Genomics. In brief, cell lysates were prepared by using a single-step proprietary lysis buffer. Lysates were incubated with a 16-gene capture array manufactured into each well of a 96-well plate. Detection was by luminescence and was performed by HTG. SDs for triplicate samples were typically 3–8% for samples with high levels of gene expression and 15–25% for samples with very low (near-threshold) levels of cytokine gene expression .
动物研究	Mouse LPS/TNF assay was performed as follows: Female CD-1 mice (8–10 weeks of age) were dosed i.v. or per os with SP600125 in PPCES vehicle (30% PEG-400/20% polypropylene glycol/15% Cremophor EL/5% ethanol/30% saline), final volume of 5 ml/kg, 15 min before i.v. injection with LPS in saline (0.5 mg/kg; Escherichia coli 055:B5). At 90 min, a terminal bleed was obtained from the abdominal vena cava, and the serum was recovered. Samples were analyzed for mouse TNF-α by using an ELISA. The in-life phase of the thymocyte apoptosis assay was performed in female C57BL/6 mice. SP600125 was administered at 0, 12, 24, and 36 h, 15 mg/kg s.c. in PPCES vehicle. Anti-CD3 (50 μg) i.p. (clone 145-2C11) was administered as a single dose immediately after SP600125 at time 0. After 48 h, mice were killed, and the thymus was dissected for thymocyte isolation. Treated and untreated mice thymuses were excised and immediately placed in complete medium (RPMI medium 1640 with 10% FBS, penicillin/streptomycin, and l-glutamine) on ice. Each thymus was then pressed between the frosted ends of 2 microscope slides to form a single cell suspension and collected through a 30 μm nylon mesh. Cells were stained for cell surface CD4 and CD8 and apoptosis and measured by flow cytometry .

储存与操作

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储存	RT
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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1,9-Pyrazoloanthrone

相似产品

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SP 600125, negative control [orb1692168]
纯度：
99.89% (May vary between batches)
CAS 号：
54642-23-8
MW:
234.25
分子式：
C₁₅H₁₀N₂O
可选规格： 50 mg, 10 mg, 25 mg, 500 mg, 100 mg
SP600125 [orb1226600]
纯度：
>98% (HPLC)
CAS 号：
129-56-6
MW:
220.2261
分子式：
C₁₄H₈N₂O
可选规格： 10 mg, 50 mg, 100 mg, 200 mg, 500 mg, 1 g
SP 600125, negative control [orb2900967]
纯度：
>98% (HPLC)
CAS 号：
54642-23-8
MW:
234.25
分子式：
C₁₅H₁₀N₂O
可选规格： 50 mg, 100 mg, 500 mg, 1 g, 5 mg, 10 mg, 25 mg
SP-600125 [orb3066672]
可选规格： 10 mg, 50 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

8515 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	99.89% (May vary between batches)
CAS 号：	54642-23-8
MW:	234.25
分子式：	C₁₅H₁₀N₂O

纯度：	>98% (HPLC)
CAS 号：	129-56-6
MW:	220.2261
分子式：	C₁₄H₈N₂O

SP-600125

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

SP 600125, negative control [orb1692168]

SP600125 [orb1226600]

SP 600125, negative control [orb2900967]

SP-600125 [orb3066672]

质量保证

化合物标识

关键属性

实验方案信息

可选规格