Y-27632 dihydrochloride

SKU: orb1307611

描述

Y-27632 dihydrochloride is a potent, cell-permeable inhibitor targeting ROCK-I and ROCK-II kinases. It is widely used in vitro to study cytoskeletal dynamics and cell adhesion, and in vivo to investigate pathways in neuroprotection, cardiovascular function, and stem cell survival.

研究领域

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号	129830-38-2
MW	320.26
纯度	99.98% (May vary between batches)
分子式	C₁₄H₂₁N₃O·2HCl
SMILES	Cl.Cl.C[C@@H](N)[C@H]1CC[C@@H](CC1)C(=O)Nc1ccncc1
靶点	ROCK,Apoptosis
溶解性	H2O:32.03 mg/mL (100.01 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (15.61 mM);DMSO:255 mg/mL (796.23 mM)

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

按应用统计成功数

反应性分布

最新实验

10 Results

稀释

样本

摘要

Organoid culture

Human

10 µM

Human ameloblastoma tissue

It was used at 10 µM...

Cell culture

Porcine

5 µM

Porcine (Sus scrofa) parthenogenetic blastocysts

It was used as a ROCK...

Cell culture

Human

5 µM

Human ESC line H9

It was use as a ROCK...

Cell culture

Human

10 µM

Human induced pluripotent stem cells

Y‑27632 is used as a ROCK...

Cell culture

Human

10 µM

hPSCs

Y‑27632 is used as a ROCK...

生物活性

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靶点 IC50	Citron kinase:5.3 μM (Ki)\|ROCK1 (p160ROCK):140 nM (Ki, cell free)\|PKN:3.1 μM (Ki)\|ROCK2:300 nM (Ki, cell free)\|PKCα:73 μM (Ki)\|PKA:25 μM (Ki)\|CITK:5.3 μM (Ki)\|ROCK1:220 nM (Ki)
体内	METHODS: To investigate the therapeutic potential of Y-27632 in motor neuron disease, Y-27632 (2 or 30 mg/kg in drinking water) was administered orally to SOD1G93A mice in the ALS model for 137 days. RESULTS: Y-27632 2 mg/kg treatment was ineffective, Y-27632 30 mg/kg treatment improved motor function in male mice, and female mice showed only limited improvement. METHODS: To investigate the effect of Y-27632 on liver fibrosis, Y-27632 (30 mg/kg) was administered orally to rats with dimethylnitrosamine (DMN)-induced liver fibrosis once a day for four weeks. RESULTS: Y-27632 treatment significantly reduced the incidence of DMN-induced hepatic fibrosis and lowered the levels of collagen and hydroxyproline as well as the expression of α-SMA in the liver.
体外	METHODS: Human induced pluripotent stem cells, marmoset iPSC, were treated with Y-27632 (5-20 μM) for 7 days and clone formation was detected by AKP. RESULTS: Y-27632 significantly improved the cloning efficiency of marmoset iPSC. METHODS: Adult adipose tissue-derived stem cells ADSCs were treated with Y-27632 (5 μmol/L) for 1 h. The morphological changes of ADSCs were detected. RESULTS: Y-27632 dose-dependently induced neuronal differentiation in ADSCs. the percentage of neuron-like cells in ADSCs treated with 5 μmol/L Y-27632 for 1 h was (93.5±4.7)%. METHODS: Crab monkey embryonic stem cells cyES were routinely passaged or treated with Y-27632 (1-10 μM) for 24 h. Live-dead staining was performed using the Flow Cytometry method, and BrdU was detected using a kit. RESULTS: Y-27632 promoted the increase of cyES surviving cells. Y-27632 did not promote cell proliferation, but protected the cells from cell death after single-cell digestion.
细胞研究	HeLa cells are plated at a density of 3×10^4 cells per 3.5-cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of the Nocodazole treatment, various concentrations of Y-27632 (0-300 μM) or vehicle is added and the cells are incubated for another 30 min .
动物研究	A group of animals was injected with a single dose of pentylenetetrazole (PTZ, 65 mg/kg) to investigate if the two Rho-kinase inhibitors, fasudil, and Y-27632, changed the onset of PTZ seizures. Fasudil, Y-27632 or saline was given intraperitoneally 30 min before the PTZ injection. Each mouse was then observed for a 15-min period to measure the onset of the first myoclonic jerk, the onset of the first clonic convulsion and the occurrence of tonic hindlimb extension. Some of the animals died after tonic hindlimb extension, which is an expected outcome of acute PTZ injection. After the observation period, all animals were killed by halothane anesthesia . Seven-week-old male Wistar rats were anesthetized with sodium pentobarbital. A silver clip (0.2 mm in diameter) was placed on the left renal artery in the preparation of the renal hypertensive rats. In the preparation of the DOCA-salt hypertensive rats, the left kidney was removed and a DOCA pellet (50 mg) was implanted subcutaneously. The DOCA rats were then fed an 8% salt diet. Rats from both groups were used after 8 weeks in the experiments, together with a male, 17–22-week old spontaneously hypertensive rats. The average systolic pressure in these groups of hypertensive rats ranged from 209 to 237 mm Hg, and no significant difference was found between groups. Eight-week-old male Wistar rats were used as controls. Their average systolic pressure was 139 mm Hg. Y-27632was administered orally. The systolic blood pressure was measured by the tail cuff method at 1, 3, 5, 7 and 24 h. The rats were prewarmed to 40 8C for 10 min before each measurement. No toxicity was found in rats treated with 30 mg kg 1 of Y-27632 administered per os once per day for 10 days .

储存与操作

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储存	Keep away from moisture, Keep away from direct sunlight,Store at low temperature, Pure form: -20°C for 3 years \| In solvent: -80°C for 1 year
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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epithelial-mesenchymal, hIPSCs, Inhibitor, inhibit, lineage, mesendodermal, orally, pluripotent, modulation, Apoptosis, ATP-competitive, active, cells, Y27632, Y-27632, Y-27632 2HCl, Y27632 Dihydrochloride, Y27632 dihydrochloride, Y-27632 Dihydrochloride, Y 27632, Y 27632 Dihydrochloride, Y 27632 dihydrochloride, Rho-kinase, ROCK1 (p160ROCK), ROCK2, ROCK, ROK, Rho-associated protein kinase, Rho-associated kinase, transition-like, stem

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Y 27632 [orb1713678]
纯度：
≥98% (HPLC)
CAS 号：
146986-50-7
MW:
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分子式：
C₁₄H₂₃Cl₂N₃O
可选规格： 1 mg, 5 mg
Y-27632 dihydrochloride [orb1226595]
纯度：
>98% (HPLC)
CAS 号：
129830-38-2
MW:
320.26
分子式：
C₁₄H₂₁N₃O·2HCl
可选规格： 5 mg, 10 mg, 25 mg, 50 mg, 100 mg, 200 mg, 500 mg, 1 g
Y-33075 dihydrochloride [orb1219732]
纯度：
>98% (HPLC)
CAS 号：
173897-44-4
MW:
353.25
分子式：
C₁₆H₁₈Cl₂N₄O
可选规格： 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 25 mg, 200 mg, 500 mg, 1 g

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化合物标识

PubChem CID

9901617 ↗

关键属性

−

暂无计算属性。

实验方案信息

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纯度：	≥98% (HPLC)
CAS 号：	146986-50-7
MW:	320.3
分子式：	C₁₄H₂₃Cl₂N₃O

纯度：	>98% (HPLC)
CAS 号：	129830-38-2
MW:	320.26
分子式：	C₁₄H₂₁N₃O·2HCl

Y-27632 dihydrochloride

描述

研究领域

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

生物活性

储存与操作

别名

相似产品

Y 27632 [orb1713678]