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描述
研究领域
Signal Transduction
图片与验证
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Item 1 of 4
| 已测应用 | ELISA, IF, IHC, WB |
|---|---|
| 稀释范围 | ELISA: 1:20,000, IHC: 20 µg/mL, IF: 1:500-1:3,000, WB: 1:500-1:3,000 |
| 反应性 | Human, Monkey, Mouse, Rat |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Mouse |
| 克隆性 | Monoclonal |
| 同种型 | IgG1 |
| 克隆号 | 17F6.B11 |
| 免疫原 | Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein. |
| 靶点 | AKT1 |
| 纯度 | Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
| 偶联 | Biotin |
储存与操作
−| 储存 | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| 形态/外观 | Lyophilized |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| 浓度 | 0.5 mg/mL |
| 有效期 | 12 months from date of receipt. |
| 免责声明 | For research use only |
别名
−mouse anti-AKT pS473 biotin conjugated Antibody, biotin conjugated mouse anti-AKT pS473 Antibody, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated
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质量保证
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