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描述
研究领域
Signal Transduction
图片与验证
−| 已测应用 | ELISA, FC, IF, IHC, WB |
|---|---|
| 稀释范围 | ELISA: User Optimized, FC: User Optimized, IHC: User Optimized, IF: User Optimized, WB: User Optimized |
| 反应性 | Human, Mouse, Rat |
| 应用说明 |
关键属性
−| 抗体类型 | Primary Antibody |
|---|---|
| 宿主 | Mouse |
| 克隆性 | Monoclonal |
| 同种型 | IgG2a |
| 克隆号 | 14E5.A2.B2.H9 |
| 免疫原 | Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development. |
| 靶点 | AKT1 |
| 纯度 | Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected. |
| 偶联 | Biotin |
储存与操作
−| 储存 | Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration. |
|---|---|
| 形态/外观 | Lyophilized |
| 缓冲液/防腐剂 | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2 |
| 浓度 | 1.0 mg/mL |
| 有效期 | 12 months from date of receipt. |
| 免责声明 | For research use only |
别名
−mouse anti-AKT1 antibody biotin conjugation, biotin conjugated mouse anti-AKT-1 antibody, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma
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