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AKT1 Antibody (Biotin)

SKU: orb344542

描述

AKT pT308 antibody (Biotin)

研究领域

Signal Transduction

图片与验证

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已测应用DOT, ELISA, IHC, WB
稀释范围ELISA: 1:20,000, IHC: 20 µg/ml, WB: 1:500 - 1:3,000
反应性Human, Monkey, Mouse, Rat
应用说明
Biotin Conjugated Anti-AKT pT308 is tested for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型IgG1
克隆号18F3.H11
免疫原Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
靶点AKT1
纯度Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research.
偶联Biotin

储存与操作

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储存Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Lyophilized
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.0 mg/mL
有效期12 months from date of receipt.
免责声明For research use only

别名

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mouse anti-AKT pT308 Biotin conjugated Antibody, Biotin conjugated mouse anti-AKT pT308 Antibody, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

UniProt 详情

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暂无 UniProt 数据

NCBI 参考序列

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相关登录号
基因转录本和蛋白产物的整理参考序列
RefSeq62241011

实验方案信息

WB
免疫印迹(WB)
查看实验方案
IHC
免疫组化
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案
DOT
斑点印迹(Dot Blot)
查看实验方案

可选规格

请在下方选择规格

50 μg
¥ 8,970.00
免费二抗(20 µl)0/0

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发货Usually dispatched within 5-10 working days
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