Biotin-16-dCTP

SKU: orb1733852

描述

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.

图片与验证

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应用说明	Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₃₂H₅₁N₈O₁₇P₃S
SMILES	C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2

储存与操作

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储存	store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Biotin-16-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

相似产品

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Item 1 of 1
Biotin-14-dCTP [orb533284]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：
C₃₁H₅₄N₇O₁₆P₃S
可选规格： 5 x 200 μl
Item 1 of 1
Biotin-16-dCTP [orb64049]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
分子式：
C₃₂H₅₁N₈O₁₇P₃S (free acid)
可选规格： 5 x 200 μl
Biotin-14-dCTP [orb1733736]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：
C₃₁H₅₄N₇O₁₆P₃S
可选规格： 200 μl

质量保证

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化合物标识

PubChem CID

176418012 ↗

关键属性

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暂无计算属性。

实验方案信息

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纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：	C₃₁H₅₄N₇O₁₆P₃S

Biotin-16-dCTP

描述

图片与验证

关键属性

储存与操作

别名

相似产品

Biotin-14-dCTP [orb533284]

Biotin-16-dCTP [orb64049]

Biotin-14-dCTP [orb1733736]

质量保证

化合物标识

关键属性

实验方案信息

可选规格