Biotin-16-dCTP

SKU: orb64049

描述

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP.

图片与验证

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Item 1 of 1

应用说明	Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

关键属性

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MW	Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
纯度	≥ 95% (HPLC)
分子式	C₃₂H₅₁N₈O₁₇P₃S (free acid)

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

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反应性分布

最新实验

9 Results

稀释

样本

摘要

Klenow fill-in reaction

Bacteria

33 μM

Linearized plasmid DNA (generated by digestion with BspQI restriction enzyme)

The primary application was to biotinylate...

sSTRIDE (sodium iodide-based transferase-independent DNA end-labeling)

Human

5 μM

Human cell lines (specifically hTERT RPE-1 cells) grown on coverslips

The primary application was in the...

Nascent Chromatin Capture (NCC) assay

Human

0.5 μM

Adherent human cell lines (specifically HLF cells, a hepatocellular carcinoma cell line)

The primary application was in the...

ARTR-seq (Assay of Reverse Transcription-based RBP binding site sequencing)

Human

0.05 mM

Fixed and permeabilized cells (HeLa, HepG2), as well as mouse embryo tissue sections

The primary application was in the...

Led-Seq (Lead Sequencing)

Bacteria

150 μM

Total RNA isolated from E. coli cells (treated with or without lead(II) acetate)

The primary application was in the...

储存与操作

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储存	store at -20 °C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Biotin-16-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl-6-aminobutanoyl)]-5-(3-propargylamino)-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

相似产品

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Item 1 of 1
Biotin-14-dCTP [orb533284]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：
C₃₁H₅₄N₇O₁₆P₃S
可选规格： 5 x 200 μl
Biotin-14-dCTP [orb1733736]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：
C₃₁H₅₄N₇O₁₆P₃S
可选规格： 200 μl
Biotin-16-dCTP [orb1733852]
纯度：
≥ 95% (HPLC)
MW:
Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid)
分子式：
C₃₂H₅₁N₈O₁₇P₃S
可选规格： 200 μl

质量保证

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化合物标识

PubChem CID

176418012 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

纯度：	≥ 95% (HPLC)
MW:	Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
分子式：	C₃₁H₅₄N₇O₁₆P₃S

Biotin-16-dCTP

描述

图片与验证

关键属性

客户验证数据

按应用统计成功数

反应性分布

最新实验

储存与操作

别名

相似产品

Biotin-14-dCTP [orb533284]

Biotin-14-dCTP [orb1733736]

Biotin-16-dCTP [orb1733852]

质量保证

化合物标识

关键属性

实验方案信息

可选规格