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DsRed Antibody

SKU: orb344410

描述

RFP antibody

图片与验证

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已测应用ELISA, WB
稀释范围ELISA: 1:75,000 - 1:150,000, WB: 1:1,000 - 1:10,000
反应性Other
应用说明
Anti-RFP antibody has been tested by ELISA, SDS-Page, and Western blot and is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP . Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

关键属性

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抗体类型Primary Antibody
宿主Mouse
克隆性Monoclonal
同种型IgG2a
克隆号8E5.G7
免疫原The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
靶点DsRed
纯度Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
偶联Unconjugated

储存与操作

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储存Store anti-RFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观Liquid (sterile filtered)
缓冲液/防腐剂Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度1.004
有效期12 months from date of receipt.
干冰运输请注意:本产品需要干冰运输,将收取干冰附加费。
免责声明For research use only

别名

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mouse anti-RFP Antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

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质量保证

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

UniProt 详情

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暂无 UniProt 数据

实验方案信息

WB
免疫印迹(WB)
查看实验方案
ELISA
酶联免疫吸附测定(ELISA)
查看实验方案

可选规格

请在下方选择规格

100 μg
选择偶联或无载体版本
免费二抗(20 µl)0/0

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