DsRed Antibody

SKU: orb345391

该产品可以定制

KO/KD
已验证

描述

RFP antibody

图片与验证

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Item 1 of 45

已测应用	ELISA, FC, IF, IHC, KO/KD Validated, WB
稀释范围	ELISA: 1:28,700 - 1:48,700, FC: 1:200 - 1:2,000, IHC: 1:200 - 1:2,000, IF: 1:200 - 1:2,000, WB: 1:1,000 - 1:5,000
反应性	Other
应用说明	Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, western blot, IF, and IHC, and is suitable for use in IP, ICC, dual RNA-FISH, iDISCO+, IEM, and FLOW. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP . Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

关键属性

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抗体类型	Primary Antibody
宿主	Rabbit
克隆性	Polyclonal
同种型	IgG
免疫原	The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
靶点	DsRed
纯度	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
偶联	Unconjugated

客户验证数据

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以下数据由各地研究人员提交。即使我们尚未自行检测，这些结果也有助于判断该产品是否适合您的应用或种属。

按应用统计成功数

反应性分布

最新实验

7 Results

稀释

样本

摘要

Mouse

1:1000

Coronal brain sections from mCherry‑tagged virus injected mouse

Sections are blocked with 4% normal...

Mouse

1:1000

Whole‑mount heart

Mouse are perfused with PFA; heart...

Mouse

1:400

Liver tissue harvested from tdTomato transgenic mice

Mouse are perfused, liver tissues are...

IHC

Mouse

1:250

Paraffin‑embedded mouse (tdTomato-tag) liver sections

After deparaffinization, antigen retrieval, and blocking...

Yeast

1:1000

mScarlet‑tagged Avl9 proteins in yeast lysates

Yeast cells are harvested, precipitated with...

储存与操作

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储存	Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
形态/外观	Liquid (sterile filtered)
缓冲液/防腐剂	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
浓度	1.03 mg/mL
有效期	12 months from date of receipt.
干冰运输	请注意：本产品需要干冰运输，将收取干冰附加费。
免责声明	For research use only

别名

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rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.

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ELISA, FC, IF, IHC, KO/KD Validated, WB
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偶联：
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应用：
ELISA, FACS, IF, IHC-Fr, IHC-P, WB
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应用：
FC, ICC, IEM, IF, IHC-Fr, IHC-P, WB
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可选规格： 20 μg, 100 μg
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应用：
ELISA, FC, ICC, IF, WB
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可选规格： 200 μg, 100 μg, 20 μg
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DsRed Antibody [orb344410]
应用：
ELISA, WB
反应性：
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克隆性：
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偶联：
Unconjugated
可选规格： 100 μg

质量保证

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