Y-27632

SKU: orb1307291

描述

ZY-27632 is a potent, cell-permeable inhibitor of ROCK-I and ROCK-II kinases. It is widely used in vitro to study cytoskeletal dynamics and cell adhesion, and in vivo to investigate its role in apoptosis prevention, such as in isolated prostate progenitor cells.

研究领域

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

图片与验证

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关键属性

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CAS 号	146986-50-7
MW	247.34
纯度	99.87% (May vary between batches)
分子式	C₁₄H₂₁N₃O
SMILES	C[C@@H](N)[C@H]1CC[C@@H](CC1)C(=O)Nc1ccncc1
靶点	ROCK,Apoptosis
溶解性	DMSO:127.5 mg/mL (515.48 mM);H2O:insoluble;10% DMSO+90% Saline:2.48 mg/mL (10.03 mM)

生物活性

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靶点 IC50	PKA:25 μM (Ki)\|PKCα:73 μM (Ki)\|PKN:3.1 μM (Ki)\|ROCK2:300 nM(Ki)\|ROCK1:220 nM(Ki)\|CITK:5.3 μM (Ki)\|Citron kinase:5.3 μM (Ki)
体内	METHODS: To investigate the therapeutic potential of Y-27632 in motor neuron disease, Y-27632 (2 or 30 mg/kg in drinking water) was administered orally to SOD1G93A mice in the ALS model for 137 days. RESULTS: Y-27632 2 mg/kg treatment was ineffective, Y-27632 30 mg/kg treatment improved motor function in male mice, and female mice showed only limited improvement. METHODS: To investigate the effect of Y-27632 on liver fibrosis, Y-27632 (30 mg/kg) was administered orally to rats with dimethylnitrosamine (DMN)-induced liver fibrosis once a day for four weeks. RESULTS: Y-27632 treatment significantly reduced the incidence of DMN-induced hepatic fibrosis and lowered the levels of collagen and hydroxyproline as well as the expression of α-SMA in the liver.
体外	METHODS: Human induced pluripotent stem cells, marmoset iPSC, were treated with Y-27632 (5-20 μM) for 7 days and clone formation was detected by AKP. RESULTS: Y-27632 significantly improved the cloning efficiency of marmoset iPSC. METHODS: Adult adipose tissue-derived stem cells ADSCs were treated with Y-27632 (5 μmol/L) for 1 h. The morphological changes of ADSCs were detected. RESULTS: Y-27632 dose-dependently induced neuronal differentiation in ADSCs. the percentage of neuron-like cells in ADSCs treated with 5 μmol/L Y-27632 for 1 h was (93.5±4.7)%. METHODS: Crab monkey embryonic stem cells cyES were routinely passaged or treated with Y-27632 (1-10 μM) for 24 h. Live-dead staining was performed using the Flow Cytometry method, and BrdU was detected using a kit. RESULTS: Y-27632 promoted the increase of cyES surviving cells. Y-27632 did not promote cell proliferation, but protected the cells from cell death after single-cell digestion.
细胞研究	Y-27632 is dissolved in water and stored. HeLa cells are plated at a density of 3×104 cells per 3.5-cm dish. The cells are cultured in DMEM containing 10% FBS in the presence of 10 mM Thymidine for 16 h. After the cells are washed with DMEM containing 10% FBS, they are cultured for an additional 8 h, and then 40 ng/mL of Nocodazole is added. After 11.5 h of the Nocodazole treatment, various concentrations of Y-27632 (0-300 μM), Y-30141, or vehicle is added and the cells are incubated for another 30 min.

储存与操作

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储存	-20°C
有效期	12 months from date of receipt.
免责声明	For research use only

别名

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Apoptosis, inhibit, Inhibitor, Y27632, Y-27632, Y 27632, Rho-associated protein kinase, Rho-kinase, Rho-associated kinase, ROK, ROCK2, ROCK1, ROCK

相似产品

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Item 1 of 2
Y-27632 2HCL [orb154626]
CAS 号：
129830-38-2
MW:
320.26
分子式：
C₁₄H₂₃Cl₂N₃O
可选规格： 100 mg, 250 mg, 1 g
Y-27632 dihydrochloride [orb1307611]
纯度：
99.98% (May vary between batches)
CAS 号：
129830-38-2
MW:
320.26
分子式：
C₁₄H₂₁N₃O·2HCl
可选规格： 5 mg, 10 mg, 25 mg, 50 mg, 100 mg, 1 ml x 10 mM (in DMSO), 1g
Y-27632 2HCl [orb1102141]
纯度：
> 98%
CAS 号：
129830-38-2
MW:
320.26
分子式：
C₁₄H₂₁N₃O.2HCl
可选规格： 20 mg
Item 1 of 1
Y 27632 [orb1713678]
纯度：
≥98% (HPLC)
CAS 号：
146986-50-7
MW:
320.3
分子式：
C₁₄H₂₃Cl₂N₃O
可选规格： 1 mg, 5 mg
Y-27632 HCl hydrate [orb1740051]
纯度：
99.98% (May vary between batches)
CAS 号：
331752-47-7
MW:
338.27
分子式：
C₁₄H₂₅Cl₂N₃O₂
可选规格： 25 mg, 1 mg, 5 mg, 10 mg

质量保证

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our 条款与条件和选购指南.

化合物标识

PubChem CID

448042 ↗

关键属性

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暂无计算属性。

实验方案信息

查看更多实验方案

按应用筛选

Cell Culture (1)
In vitro (1)

按种属筛选

Human (2)

Jane In den Birken et al. Investigating the Anticancer Effects of Sulforaphane in an In Vitro Coculture Model of Prostate Cancer Cells with Engineered Heart Tissue ACS Pharmacol Transl Sci, (2025)

PubMed: 41409171

Nicola Wanner et al. Lysine-specific histone demethylase 1a regulates nephron development and long-term transcriptional programming JCI Insight, 11(5), e190283 (2026)

PubMed: 41797715

应用

Cell Culture

反应性

Human

Anne Strigli, Christian Raab, Sabine Hessler, Tobias Huth, Adam J. T. Schuldt, Christian Alzheimer, Thomas Friedrich, Paul W. Burridge, Mark Luedde, Michael Schwake Doxorubicin induces caspase-mediated proteolysis of KV7.1 bioRxiv, 28, 155 (2018)

PubMed: 30302399

应用

In vitro

反应性

Human

CAS 号：	129830-38-2
MW:	320.26
分子式：	C₁₄H₂₃Cl₂N₃O

纯度：	99.98% (May vary between batches)
CAS 号：	129830-38-2
MW:	320.26
分子式：	C₁₄H₂₁N₃O·2HCl

纯度：	> 98%
CAS 号：	129830-38-2
MW:	320.26
分子式：	C₁₄H₂₁N₃O.2HCl

纯度：	≥98% (HPLC)
CAS 号：	146986-50-7
MW:	320.3
分子式：	C₁₄H₂₃Cl₂N₃O

Y-27632

描述

研究领域

图片与验证

关键属性

生物活性

储存与操作

别名

相似产品

Y-27632 2HCL [orb154626]

Y-27632 dihydrochloride [orb1307611]

Y-27632 2HCl [orb1102141]

Y 27632 [orb1713678]

Y-27632 HCl hydrate [orb1740051]

质量保证

化合物标识

关键属性

实验方案信息

可选规格