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I tested the antibody on human ovarian cancer cell extracts and A549 cell line. Samples were collected, sonicated, mixed with loading buffer, and resolved on a vertical electrophoresis system using denaturating conditions. I incubated the nitrocellulose membrane using 1:1000 antibody diluted in 5% non-fat milk with TBS-T for 2 h on rt, washed in TBS-T and incubated with adequate secondary antibody. Visualization was done using ECL. Signals were strong, but there was three of them, between the…

反应性:Human
应用:WB
Juran KraljRuđer Bošković Institute, Zagreb, Croatia
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Performs well! I used P-mtor in my western blot protocole The probing of p mtor is a little bad and didnt give clear bands after revelations After probing the membrane many times,its give a clear bands but with a little background.

反应性:Human
应用:WB
Bariaa NafehLebanese University
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I found it very effective antibody against the diabetes, it is easy to use, it's cost is normal, I recommend other people to use it if you want be healthy.

反应性:Rat
应用:IHC
Gada Hussain SuhagNULL
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MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis. Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

反应性:Human
应用:ELISA
Sameer Srivastav
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I used the Biorbyt IL-17RE antibody (orb184432) for the immunofluorescence in the epithelial cells. The results come back fantastic and the staining was bright and specific.

反应性:Human
应用:IF
Jianfeng LinThe Lundquist Institute
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I used this to target the phenotype antibody secretory cells (CD27hi CD38hi) inside the memory B cells population. Extremely happy with the results. I use a lower concentration than the manufacturer says cause this is a bright antibody. Definitely, one of the best combinations ever.Performs extremely well in low activity population

反应性:Human
应用:FC
Sara SanzIMIM - Hospital del Mar
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Highly sensitive, and reproducible results were obtained for multiple independent experiments. Quality and batch to batch consistency is excellent. Can be used very effectively for multiple applications.

反应性:Human
应用:FC
Suchita Bhattacharyyahuman
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Boiling of cell lysates and tissue extracts, western blot, expression studies. Great reproducibility. Good and clear results. Excellent product, will definitely buy again.

反应性:Human
应用:WB
Para ShaUniversity of Louisville
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Analyse cell for immune response during infection with infectious agents. The product is great and wonderful to use. It is good!

反应性:Human
应用:IHC
Adeyemi OlanrewajuGeorge Mason University
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