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For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.

反应性:Human
应用:IP, IF, WB
Tanisha SharmaNCCS
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CLK1 Antibodyorb126821

Works well at 1:500 with 2% PFA fix in HeLa cells. Also picks up endogenous CLK1 in WB at 1:1000.

反应性:Human
应用:WB, IF
Lauren WilliamsUniversity of Utah
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The results are reproducible and the product works very well if the protocols are standardized. Performs very well.

反应性:Human
应用:WB
Rahul Gupta
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High-quality product with clear and reproducible results in human FC with CD206 antibody orb4941.

反应性:Human
应用:FC
Bhagwan RekadwadSavitribai Phule Pune University
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"The antibodies worked really well and have great reproducibility. Great value for the cost." Review date: 27 Jun 2019 https://www.selectscience.net/products/cd206-antibody/?prodID=210699

反应性:Human
应用:WB
John KyndtBellevue University
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The antibody works well on sheep liver tissue, although background is found even using avidin-biotin blocking. Apart from this, the antibody label specifically macrophages. Performs well without variability.

反应性:Sheep
应用:IHC
Veronica MolinaUniversity of Cordoba
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GFAP antibody works great on mouse spinal frozen sections! Our immunofluorescence signal is specific, astrocytes show distinct morphology, and the staining effect is very beautiful!

Customer's review image 1 for orb1294403
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反应性:Mouse
应用:IF
Customer
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