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Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones. Not bad for screening knockouts, but there is a nonspecific band close to the expected band which complicates analysis. I think the lower band is the correct band, though I can't be sure.

Tamanna Hossin's review image 1 for orb156964
反应性:Mouse
应用:WB
Tamanna HossinYale, Tech
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It is a good cell-surface blocking antibody(HTF1). I used it in one-stage and two-stage clotting assays with the conc. of 10 ug/ml in Human endothelial cells.

Saravanan Subramaniam's review image 1 for orb402784
反应性:Human
应用:Activity Assay
Saravanan SubramaniamVersiti-Blood Research Institute, Research Scientist
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We have used this CD133 antibody for quite some time, as it gives us highly reproducible results and easily identified staining. The concentration ratio allows us to do multiple sets of slides with the same lot and produces publication-quality results every time." Review date: 04 Sep 2020

反应性:Mouse
应用:IHC
Catherine Langford
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We performed CHIP-SICAP, a modified CHIP protocol that involves stringent wash to remove non-specific chromatin-associated proteins. So, labeling DNA with biotin followed by steptavidin is key to achieving high sensitivity. We evaluated the results by qPCR. We got great results with as little as four times of the cells as prescribed by other CHIP SICAP users.

反应性:Other
应用:ChIP
Charlesantony Aruljothi
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The product seems to be reliable and confers robust results, with reproductivity. The pricing is fair, although import fee and shipping times can be a nuisance. Good product overall.

反应性:Human
应用:WB
Ricardo SoaresUniversidade de São Paul
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The IFNAR1 antibody (orb5499) was effective and easy to titrate for accurate results in human FC test.

反应性:Human
应用:FC
CustomerUniversity of Miami
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"Great quality product. Antibody very high quality, achieved very high publication quality gels, no specificity issues and the protocol was spot on! Worked first time no optimization required."

反应性:Mouse
应用:WB
Alison DicksonSwansea University
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The chemical easily labeled nascent peptides even at lower concentrations. I have repeated the experiments three times, and the results are consistent.

反应性:Other
应用:Conjugation
Jie Tian
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